M17H6

Prompt

Adenylate kinase is involved in the metabolic process of purine ribonucleoside monophosphates. This enzyme contains a conserved site essential for its function.

AI Generated Sequence

189 AAs | 21.13 kDa | 22.95 kDa (with tag)

MPRFVVVTGIPGVGKTTLVAQEALERTGERFKLVNFGNVMFEEAAKEGLVAHRDEMRKLPLETQRQLQKAAAGRIVEVAREGPVVVDTHASIKTPHGYLPGLPPEVLERLNPDLVVVIEADPQEILGRRLRDETRHRDIETEEQIERHEQMNRAASMAYAVMTGAVIKIVENRDGKEEEAVNELLAVLE

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

68.97

AAComp

88.61

DiffDock Confidence

-0.62

Seqdif (SeqID)

63.16 (WP_013747832)

Structdif (F-Target)

96.06 (3h86 6)

ProtNLM

Name	Score
AK	0.324
Adenylate kinase	0.265
2.7.4.3	0.207
ATP-AMP transphosphorylase	0.196
Adenylate monophosphate kinase	0.002
ATP:AMP phosphotransferase	0.001
AAA family ATPase	0.000
Nucleoside monophosphate kinase	0.000
ADK_lid domain-containing protein	0.000
Dual activity adenylate kinase/ATPase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.85 (Lunatic)	42.5	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.98 (Lunatic)	49	100	N/A	N/A
Tierra	T3	Biologics Optimized System	1.15 (Lunatic)	57.5	95.5	N/A	N/A
Tierra	T4	Biologics Optimized System	0.61 (Lunatic)	30.5	100	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

7atp_-0.65 | 7