MKCPT

Prompt

The protein is part of the ABC transporter superfamily and functions in ATPase dependent transmembrane transport. It facilitates the movement of substances across cellular membranes using ATP energy.

AI Generated Sequence

222 AAs | 24.25 kDa | 26.07 kDa (with tag)

MIELRNVSKSYGDTPVLKGVSLTVPAGEIVGVLGPSGSGKSTLLRMLNGLEPYTSGEILIDGKDVTKMPPVEVRRGIGYVLQEPRLIFPLTVRENVAFPLRRGVKKAEAEARVRELLEMVGLKDKADKYPGQLSGGQQQRVAIARALVTKPKLLLLDEPFSGLDPLLVLEMQELIRRLKEEGITIVFVTHDVNEALELADRVVVMKNGKIVADGTPEELLAD

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

85.28

AAComp

90.63

DiffDock Confidence

-1.09

Seqdif (SeqID)

52.25 (MBR2836032)

Structdif (F-Target)

93.82 (2pcj B)

ProtNLM

Name	Score
ABC transporter ATP-binding protein	0.240
ATP-binding cassette domain-containing protein	0.155
Osmoprotectant transport system ATP-binding pr...	0.054
Proline/glycine betaine ABC transporter ATP-bi...	0.050
Amino acid ABC transporter ATP-binding protein	0.049
Glycine/betaine ABC transporter ATP-binding pr...	0.048
L-proline glycine betaine ABC transport system...	0.034
ABC transporter related	0.028
ABC transporter domain-containing protein	0.027
ABC transporter related protein	0.022

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.39 (Lunatic)	19.5	90.1	N/A	N/A
Tierra	T4	Enzyme Optimized System	1.69 (Lunatic)	84.5	96	N/A	N/A
Tierra	T3	Biologics Optimized System	0.32 (Lunatic)	16	91	N/A	N/A
Tierra	T4	Biologics Optimized System	1.52 (Lunatic)	76	97.2	N/A	N/A
Arctoris	N/A	E. coli	N/A	N/A	N/A	N/A	N/A

Data

11atp_-1.07t | 24