M29OT

Prompt

1.14.12.25

Oxidoreductases. Acting on paired donors, with incorporation or reduction of molecular oxygen. The oxygen incorporated need not be derived from O2. With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into one donor. p-cumate 2,3-dioxygenase.

AI Generated Sequence

170 AAs

MENLKKKKLVVVGGGLAGVELAAELHDFAREDLCKLYPNVKELVRITLIEAGPRILAPFDERISAFAEEQLKDRGVDIRLGSRVMKVEADHVVMKDGSVAYDYLVMAVGSVTNTFGIPGVKEHCYFLKEVSDAQKIRNRVIDCFLRAELPGTTDEERRRLLSFVIVGGGA

Ligand/Cofactor

NADH

C1C=CN(C=C1C(=O)N)C2C(C(C(O2)COP(=O)(O)OP(=O)(O)OCC3C(C(C(O3)N4C=NC5=C(N=CN=C54)N)O)O)O)O

Validation Metrics

PLDDT	86.8
NLMSIM	70.0
AACOMP	91.6
Seqdif (SeqID)	51.2 (CAE6450985.1)
Structdif (F-Target)	31.4 (3h8l B)

ProtNLM

Name	Score
Pyr_redox_2 domain-containing protein	0.844
FAD-dependent oxidoreductase	0.063
Pyridine nucleotide-disulfide oxidoreductase	0.035
NAD(P)/FAD-dependent oxidoreductase	0.016
NADH dehydrogenase	0.005
FAD-dependent pyridine nucleotide-disulfide oxidoreductase	0.003