MBMLF

Prompt

This protein has adenylate kinase activity and is involved in the metabolic process of ribonucleoside monophosphates.

AI Generated Sequence

214 AAs | 23.29 kDa | 25.11 kDa (with tag)

MNIILLGPPGAGKGTQAKRIVEKYGIPQISTGDMLRAAVAAGTELGKKAKAVMDAGELVPDEITIGIVEERIAQDDCKKGFILDGFPRTVAQAEALDAMLADLGKKIDHVININVDESVLVERITGRFIHKNSGATYHIEFNPPKVEGVDDKTGGELFQRADDKEETVKTRLEVYMKQTEPLLDFYEAKGILRNIDGQKDIDKVFADIKAVLGG

Ligand/Cofactor

Adenosine triphosphate

C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N

Computational Metrics

PLDDT

GPT Score

70.73

AAComp

90.18

DiffDock Confidence

-1.59

Seqdif (SeqID)

73.83 (5G40_A)

Structdif (F-Target)

98.03 (2oo7 A)

ProtNLM

Name	Score
AK	0.237
Adenylate kinase	0.189
Adenylate monophosphate kinase	0.150
2.7.4.3	0.145
ATP:AMP phosphotransferase	0.141
ATP-AMP transphosphorylase	0.133
ADK_lid domain-containing protein	0.000
Nucleoside monophosphate kinase	0.000
Polyphosphate kinase	0.000
Guanylate kinase	0.000

Experimental Results

Company	Variant	Expression System	Concentration (mg/ml)	Total Yield (µg)	Purity (%)	TM (°C)	Tm(oC w/2mM AMP-PNP)
Tierra	T3	Enzyme Optimized System	0.05 (Lunatic)	2.5	100	N/A	N/A
Tierra	T4	Enzyme Optimized System	0.61 (Lunatic)	30.5	100	N/A	N/A
Tierra	T3	Biologics Optimized System	0.17 (Lunatic)	8.5	100	N/A	N/A
Tierra	T4	Biologics Optimized System	0.36 (Lunatic)	18	100	N/A	N/A
Arctoris	N/A	E. coli	0.538 (A660nm)	69.94	>95	56.4	57.1

Data

59_-1.18 | 95